Diagnostics of the biochemical alterations in packed human red blood cells (PRBCs) with the use of vibrational spectroscopy


                

Project manager:

Realised by:

Grant no.:

  • LIDER/13/0076/L-8/16/NCBR/2017

Start date:

  • 2018-03-01

End date:

  • 2021-07-01

A large number of patients worldwide are in need of periodic, therapeutic transfusions of packed red blood cells (PRBCs). This has led to an increased demand for an efficient technology providing information on the PRBCs alterations, which may affect their quality. The goal of this project is the development of the spectroscopic methodology for the Raman (RS) and Infrared (IR) diagnosis of the pre-transfusion quality of RBCs. In this project we are planning to study PRBCs fractions with the focus on biochemical changes of RBCs due to donor characteristics, age and characteristics of PRBCs, level of oxidative stress and damage in RBCs (steps I and II). RS and IR spectroscopies will provide fast determination of the RBCs condition before transfusions. To understand the biochemistry behind all changes observed by IR and RS, the RBCs samples will be studied also by other classical techniques. We can include here absorption spectrophotometry, blood gas analysis, flow cytometry, atomic force microcopy, ektocytometry, staining’s and the measurement of the chosen biochemical parameters. The focus on comprehensive assessment of PRBCs will provide the knowledge about biochemical fingerprints of RBCs condition. The last step of project (step III), which goal is an evaluation of the obtained SPECDIAG-RED methodology, will be focused on the studies of previously chosen spectroscopic biomarkers of biochemical state of PRBCs for a specific group of patient (patients with acute myeloid or/and acute lymphoblastic leukemia). The project will not affect the medical procedures performed routinely in hospital as the IR/RS measurement will be carry out on the small sample of PRBCs (1 mL) or by the direct measurement through the PRBCs wall. The PRBCs state will be assessed with the use of SPEC-DIAG-RED methodology and combine with the feedback from the clinics about patients condition before and after transfusion what will allow for the final evaluation of results.

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